                         SEQUENCE LISTING

<110>  SANGAMO BIOSCIENCES, INC.
       COLLINGWOOD, Trevor
       GREGORY, Philip D.
 
<120>  METHODS AND COMPOSITIONS FOR GENERATION OF BAX- AND BAK-DEFICIENT
        CELL LINES

<130>  8325-0062.40

<140>  PCT/US2009/003484
<141>  2009-06-10

<150>  US 61/131,579
<151>  2008-06-10

<150>  US 61/133,792
<151>  2008-07-02

<160>  94    

<170>  PatentIn version 3.5

<210>  1
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  1

Arg Ser Asp His Leu Ser Thr 
1               5           


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  2

Asp Arg Ser His Leu Ala Arg 
1               5           


<210>  3
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK/BAX

<400>  3

Gln Ser Ser His Leu Thr Arg 
1               5           


<210>  4
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  4

Arg Ser Asp Asn Leu Arg Glu 
1               5           


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  5

Arg Ser Ala Asn Leu Ser Val 
1               5           


<210>  6
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  6

Asp Arg Ala Asn Leu Ser Arg 
1               5           


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK/BAX

<400>  7

Asn Arg Thr Asp Leu Ile Arg 
1               5           


<210>  8
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK/BAX

<400>  8

Thr Ser Ser Asn Leu Ser Arg 
1               5           


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  9

Arg Ser Asp His Leu Thr Thr 
1               5           


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  10

Arg Ser Asp His Leu Ser Glu 
1               5           


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  11

Arg Asn Asp Asn Arg Lys Thr 
1               5           


<210>  12
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  12

Gln Ser Gly His Leu Gln Arg 
1               5           


<210>  13
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  13

Gln Ser Gly His Leu Ser Arg 
1               5           


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  14

Arg Ser Asp Ser Leu Ser Ala 
1               5           


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  15

Asp Arg Ser Ser Arg Thr Lys 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  16

Arg Ser Asp Asp Leu Thr Arg 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  17

Arg Ser Asp Ala Leu Ala Arg 
1               5           


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker sequence

<400>  18

Thr Gly Glu Lys Pro 
1               5   


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker sequence

<400>  19

Thr Gly Gln Lys Pro 
1               5   


<210>  20
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker sequence

<400>  20

Thr Gly Ser Gln Lys Pro 
1               5       


<210>  21
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 10077

<400>  21
cagggtggct gg                                                           12


<210>  22
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 10079

<400>  22
gatccagaca ag                                                           12


<210>  23
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 11205

<400>  23
ggaggaaagg ggtgg                                                        15


<210>  24
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 10350

<400>  24
gtggcggccc tg                                                           12


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  GJC 39F

<400>  25
tcaagaggtt tcatggcgag                                                   20


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  GJC 38R

<400>  26
ttctctctct tgtgcttatg g                                                 21


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  GJC 24F

<400>  27
catctcacat ctggaccaca gccg                                              24


<210>  28
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  GJC 25R

<400>  28
ctggaactct gtgtcgtatc tccgg                                             25


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  GJC 12F

<400>  29
cttcttccgg gtggcagctg                                                   20


<210>  30
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  GJC 23R

<400>  30
cccgaagtat gagaggaggc catc                                              24


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  GJC 52F

<400>  31
cagaggaatg aaagcaaagg                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  GJC 114R

<400>  32
tgaaccaggc tgggagattt                                                   20


<210>  33
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 14238

<400>  33
tttcaggcct tggttggc                                                     18


<210>  34
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  34

Asp Arg Ser His Leu Ser Arg 
1               5           


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  35

Thr Ser Gly Ser Leu Thr Arg 
1               5           


<210>  36
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  36

Arg Ser Asp Asn Leu Ser Glu 
1               5           


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  37

His Ser Asn Ala Arg Lys Thr 
1               5           


<210>  38
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 10526

<400>  38
cgtctggaca ag                                                           12


<210>  39
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  39

Arg Ser Asp Asn Leu Ser Val 
1               5           


<210>  40
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  40

Asp Arg Ser Asn Leu Thr Arg 
1               5           


<210>  41
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  41

Arg Ser Asp Thr Leu Ser Glu 
1               5           


<210>  42
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  42

Arg Ser Gln Thr Arg Lys Thr 
1               5           


<210>  43
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 17658

<400>  43
gtcctggatc cagcc                                                        15


<210>  44
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 17659

<400>  44
ggttgggtga ggcctg                                                       16


<210>  45
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  45

Asp Arg Ser Asp Leu Ser Arg 
1               5           


<210>  46
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  46

Arg Ser Asp Thr Leu Ser Gln 
1               5           


<210>  47
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  47

Asp Arg Ser Ala Arg Thr Arg 
1               5           


<210>  48
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  48

Arg Ser Asp Ala Leu Ser Val 
1               5           


<210>  49
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  49

Asp Ser Ser His Arg Thr Arg 
1               5           


<210>  50
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK/BAX

<400>  50

Gln Asn Ala His Arg Lys Thr 
1               5           


<210>  51
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAX

<400>  51

Thr Ser Gly His Leu Ser Arg 
1               5           


<210>  52
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 17622

<400>  52
aatgatgcag catga                                                        15


<210>  53
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  53

Gln Ser Gly Asp Leu Thr Arg 
1               5           


<210>  54
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  54

Gln Ser Ser Asn Leu Ala Arg 
1               5           


<210>  55
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  55

Thr Ser Ser Asn Arg Lys Thr 
1               5           


<210>  56
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 17623

<400>  56
ccggtggatc gcgcagag                                                     18


<210>  57
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  57

Arg Ser Asp Asn Leu Ala Arg 
1               5           


<210>  58
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  58

Asp Asn Arg Gln Leu Ile Thr 
1               5           


<210>  59
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  59

Arg Ser Asp Thr Leu Ser Arg 
1               5           


<210>  60
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  60

Arg Asn Asp Asp Arg Ile Thr 
1               5           


<210>  61
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 17627

<400>  61
aggggtggct gggtag                                                       16


<210>  62
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  62

Arg Ser Asp Asn Leu Thr Thr 
1               5           


<210>  63
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  63

Arg Ser Asp His Leu Ser Arg 
1               5           


<210>  64
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  64

Gln Ser Ser Asp Leu Arg Arg 
1               5           


<210>  65
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  65

Arg Ser Asp His Leu Thr Gln 
1               5           


<210>  66
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  SBS 17626

<400>  66
gatccaccgg gcaatgca                                                     18


<210>  67
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  67

Gln Ser Gly Ser Leu Thr Arg 
1               5           


<210>  68
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  68

Asp Arg Ser His Leu Thr Arg 
1               5           


<210>  69
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic zinc finger nuclease targeted to BAK

<400>  69

Arg Ser Asp Asp Arg Lys Thr 
1               5           


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  YS 13F

<400>  70
ctcctttcac agagatgcag                                                   20


<210>  71
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  YS 14R

<400>  71
caggagagac agagtggtca                                                   20


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  YS 21F

<400>  72
aaaaagactg cagtggcgca                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  YS 22R

<400>  73
tcacccagag gtcaatggat                                                   20


<210>  74
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide:  linker sequence

<400>  74

Thr Gly Gly Gln Arg Pro 
1               5       


<210>  75
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 1:  39F\38R

<400>  75
cagtgctgcc aaccaaggcc tgaaagatgg cgtctggaca aggacc                      46


<210>  76
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 1:  Bak 8H6-1/Bak 8H6-3

<400>  76
cagtgctgga caaggacc                                                     18


<210>  77
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 1:  Bak 8H6-2/Bak 8H6-4

<400>  77
cagtgctgcc aaccaaggcc catctggaca aggacc                                 36


<210>  78
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 1:  Bak 8D4-1


<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  n is a, c, g, or t

<400>  78
cagtgntgcc aaccaaggac aaggacc                                           27


<210>  79
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 1:  Bak 8D4-2/Bak 8D4-3/Bak 8D4-4

<400>  79
cagtgctgcc aaccaaggac aaggacc                                           27


<210>  80
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  52F114R

<400>  80
tggacactgg acttcctccg agagcggctg cttgtctgga tccaagacca gggtggctgg       60

gtgagacccc ttagtccttg tcacacttta gactagtggt tctcaaactt c               111


<210>  81
<211>  115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  11, 18, 22, 28, 50, and 91

<400>  81
tggacactgg acttcctccg agagcggctg cttgtctgga tccaagaaag accagggtgg       60

ctgggtgaga ccccttagtc cttgtcacac tttagactag tggttctcaa acttc           115


<210>  82
<211>  112
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  13

<400>  82
tggacactgg acttcctccg agagcggctg cttgtctgga tccaagaacc agggtggctg       60

ggtgagaccc cttagtcctt gtcacacttt agactagtgg ttctcaaact tc              112


<210>  83
<211>  108
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  30

<400>  83
tggacactgg acttcctccg agagcggctg cttgtctgga ttgacgcaga taaaattcca       60

tttcatccct actatactat taaagaccta ctaggagcct tcatgcta                   108


<210>  84
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  35

<400>  84
tggacactgg acttcctccg agagcggctg cttgtctgga tccaagagac tctgctggcg       60

ctctaccttg tccctcatgt tctgtgtggc tttagggaag aaccctttat                 110


<210>  85
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  40, 87

<400>  85
tggacactgg acttcctccg agagcggctg cttgtctgga tccagggtgg ctgggtgaga       60

ccccttagtc cttgtcacac tttagactag tggttctcaa acttc                      105


<210>  86
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  46

<400>  86
ggtgagctgg gtgagacccc ttagtccttg tcacacttta gactagtggt tctcaaactt       60

c                                                                       61


<210>  87
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  47

<400>  87
tggacactgg acttcctccg agagcggctg cttgtctgga tccagactag tggttctcaa       60

acttc                                                                   65


<210>  88
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  57

<400>  88
tggacactgg acttcctccg agagcggctg cttgtctgga tccaagaaga ccagggtggc       60

tgggtgagac cccttagtcc ttgtcacact ttagactagt ggttctcaaa cttc            114


<210>  89
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  71

<400>  89
tggacactgg acttcctccg agagcggctg cttgtcaaac ttc                         43


<210>  90
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  72

<400>  90
tggacactgg acttcctccg agagcggctg cttgtctgga tccatgaggg cagagtatct       60

acagaaatat ttgtaatctt tctactgata atactcatct cttcctaact catg            114


<210>  91
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  93


<220>
<221>  misc_feature
<222>  (51)..(51)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (74)..(74)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (91)..(91)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (96)..(96)
<223>  n is a, c, g, or t

<400>  91
tggacactgg acttcctccg agagcggctg cttgtctgga tccaagacca nggtggctgg       60

gtgagacccc ttantccttg tcacacttta nactantggt tctcaaactt c               111


<210>  92
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  97

<400>  92
tggacactgg acttcctcc                                                    19


<210>  93
<211>  86
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  106

<400>  93
tggacactgg acttcctccg agagcggctg cttgtctgag accccttagt ccttgtcaca       60

ctttagacta gtggttctca aacttc                                            86


<210>  94
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence from Fig. 4B:  108

<400>  94
acactggact tcctccgaga gcggctgctt gtctggatcc aagataagac cagggtggct       60

gggtgagacc ccttagtcct tgtcacactt tagactagtg gttctcaaac ttc             113


